Polymorphisms in the Mitochondrial Genome Are Associated With Bullous Pemphigoid in Germans.

TitelPolymorphisms in the Mitochondrial Genome Are Associated With Bullous Pemphigoid in Germans.
MedientypJournal Article
Jahr der Veröffentlichung2019
AutorenRusslies J, Fähnrich A, Witte M, Yin J, Benoit S, Gläser R, Günter C, Eming R, Erdmann J, Gola D, Gupta Y, Holtsche MMarleen, Kern JS, König IR, Kiritsi D, Lieb W, Sadik CD, Sárdy M, Schauer F, van Beek N, Weidinger A, Worm M, Zillikens D, Schmidt E, Busch H, Ibrahim SM, Hirose M
JournalFront Immunol
Volume10
Pagination2200
Datum der Veröffentlichung2019
ISSN1664-3224
Zusammenfassung

Bullous pemphigoid (BP) is the most prevalent autoimmune skin blistering disease and is characterized by the generation of autoantibodies against the hemidesmosomal proteins BP180 (type XVII collagen) and BP230. Most intriguingly, BP is distinct from other autoimmune diseases because it predominantly affects elderly individuals above the age of 75 years, raising the question why autoantibodies and the clinical lesions of BP emerges mostly in this later stage of life, even in individuals harboring known putative BP-associated germline gene variants. The mitochondrial genome (mtDNA) is a potential candidate to provide additional insights into the BP etiology; however, the mtDNA has not been extensively explored to date. Therefore, we sequenced the whole mtDNA of German BP patients ( = 180) and age- and sex-matched healthy controls ( = 188) using next generation sequencing (NGS) technology, followed by the replication study using Sanger sequencing of an additional independent BP ( = 89) and control cohort ( = 104). While the BP and control groups showed comparable mitochondrial haplogroup distributions, the haplogroup T exhibited a tendency of higher frequency in BP patients suffering from neurodegenerative diseases (ND) compared to BP patients without ND (50%; 3 in 6 BP with haplogroup T). A total of four single nucleotide polymorphisms (SNPs) in the mtDNA, namely, m.16263T>C, m.16051A>G, and m.16162A>G in the D-loop region of the mtDNA, and m.11914G>A in the mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 4 gene (), were found to be significantly associated with BP based on the meta-analysis of our NGS data and the Sanger sequencing data ( = 0.0017, = 0.0129, = 0.0076, and = 0.0132, respectively, Peto's test). More specifically, the three SNPs in the D-loop region were negatively, and the SNP in the gene was positively associated with BP. Our study is the first to interrogate the whole mtDNA in BP patients and controls and to implicate multiple novel mtDNA variants in disease susceptibility. Studies using larger cohorts and more diverse populations are warranted to explore the functional consequences of the mtDNA variants identified in this study on immune and skin cells to understand their contributions to BP pathology.

DOI10.3389/fimmu.2019.02200
Alternate JournalFront Immunol
PubMed ID31824475
PubMed Central IDPMC6883920