Titel | Consistent alterations in faecal microbiomes of patients with primary sclerosing cholangitis independent of associated colitis. |
Medientyp | Journal Article |
Jahr der Veröffentlichung | 2019 |
Autoren | Rühlemann M, Liwinski T, Heinsen F-A, Bang C, Zenouzi R, Kummen M, Thingholm L, Tempel M, Lieb W, Karlsen T, Lohse A, Hov J, Denk G, Lammert F, Krawczyk M, Schramm C, Franke A |
Journal | Aliment Pharmacol Ther |
Volume | 50 |
Ausgabe | 5 |
Pagination | 580-589 |
Datum der Veröffentlichung | 2019 Sep |
ISSN | 1365-2036 |
Zusammenfassung | <p><b>BACKGROUND: </b>Single-centre studies reported alterations of faecal microbiota in patients with primary sclerosing cholangitis (PSC). As regional factors may affect microbial communities, it is unclear if a microbial signature of PSC exists across different geographical regions.</p><p><b>AIM: </b>To identify a robust microbial signature of PSC independent of geography and environmental influences.</p><p><b>METHODS: </b>We included 388 individuals (median age, 47 years; range, 15-78) from Germany and Norway in the study, 137 patients with PSC (n = 75 with colitis), 118 with ulcerative colitis (UC) and 133 healthy controls. Faecal microbiomes were analysed by 16S rRNA gene sequencing (V1-V2). Differences in relative abundances of single taxa were subjected to a meta-analysis.</p><p><b>RESULTS: </b>In both cohorts, microbiota composition (beta-diversity) differed between PSC patients and controls (P < 0.001). Random forests classification discriminated PSC patients from controls in both geographical cohorts with an average area under the curve of 0.88. Compared to healthy controls, many new cohort-spanning alterations were identified in PSC, such as an increase of Proteobacteria and the bile-tolerant genus Parabacteroides, which were detected independent from geographical region. Associated colitis only had minor effects on microbiota composition, suggesting that PSC itself drives the faecal microbiota changes observed.</p><p><b>CONCLUSION: </b>Compared to healthy controls, numerous microbiota alterations are reproducible in PSC patients across geographical regions, clearly pointing towards a microbiota composition that is shaped by the disease itself and not by environmental factors. These reproducibly altered microbial populations might provide future insights into the pathophysiology of PSC.</p> |
DOI | 10.1111/apt.15375 |
Alternate Journal | Aliment. Pharmacol. Ther. |
PubMed ID | 31250469 |
Grant List | EXC306 / / Deutsche Forschungsgemeinschaft / EXC306/2 / / Deutsche Forschungsgemeinschaft / KFO306 / / Deutsche Forschungsgemeinschaft / SFB1182 / / Deutsche Forschungsgemeinschaft / / / YAEL-Foundation / 2016067 / / Helse Sør-Øst RHF / / / Helmut and Hannelore Greve Foundation / 01ZX1306A / / Bundesministerium für Bildung und Forschung / 240787/F20 / / Norges Forskningsråd / |