Identification and characterization of two functional variants in the human longevity gene FOXO3.

TitelIdentification and characterization of two functional variants in the human longevity gene FOXO3.
MedientypJournal Article
Jahr der Veröffentlichung2017
AutorenFlachsbart F, Dose J, Gentschew L, Geismann C, Caliebe A, Knecht C, Nygaard M, Badarinarayan N, ElSharawy A, May S, Luzius A, Torres GG, Jentzsch M, Forster M, Häsler R, Pallauf K, Lieb W, Derbois C, Galan P, Drichel D, Arlt A, Till A, Krause-Kyora B, Rimbach G, Blanché H, Deleuze J-F, Christiansen L, Christensen K, Nothnagel M, Rosenstiel P, Schreiber S, Franke A, Sebens S, Nebel A
JournalNat Commun
Datum der Veröffentlichung2017 12 12
SchlüsselwörterAge Factors, Aged, Aged, 80 and over, Alleles, CCCTC-Binding Factor, Computer Simulation, European Continental Ancestry Group, Female, Forkhead Box Protein O3, Haplotypes, Humans, Insulin-Like Growth Factor I, Introns, Longevity, Male, Middle Aged, Polymorphism, Single Nucleotide, RNA, Messenger, Serum Response Factor

<p>FOXO3 is consistently annotated as a human longevity gene. However, functional variants and underlying mechanisms for the association remain unknown. Here, we perform resequencing of the FOXO3 locus and single-nucleotide variant (SNV) genotyping in three European populations. We find two FOXO3 SNVs, rs12206094 and rs4946935, to be most significantly associated with longevity and further characterize them functionally. We experimentally validate the in silico predicted allele-dependent binding of transcription factors (CTCF, SRF) to the SNVs. Specifically, in luciferase reporter assays, the longevity alleles of both variants show considerable enhancer activities that are reversed by IGF-1 treatment. An eQTL database search reveals that the alleles are also associated with higher FOXO3 mRNA expression in various human tissues, which is in line with observations in long-lived model organisms. In summary, we present experimental evidence for a functional link between common intronic variants in FOXO3 and human longevity.</p>

Alternate JournalNat Commun
PubMed ID29234056
PubMed Central IDPMC5727304
Grant ListP01 AG008761 / AG / NIA NIH HHS / United States